Wednesday, October 3, 2012

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, has been deployed in a number of model organisms and can reliably detect three or more action potentials in short bursts in several systems in vivo.
Akerboom J et al.
J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 10.1523/JNEUROSCI.2601-12.2012.

Tian L et al.
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators.
Nat Methods. 2009 Dec;6(12):875-81. doi: 10.1038/nmeth.1398. Epub 2009 Nov 8.

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